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| Gonçalves, Edilaine Della Valentina; Stangarlin, José Renato; Roncato, Sidiane Coltro; Dildey, Omari Dangelo Forlin; Meinerz, Cristiane Cláudia; Kuhn, Odair José. |
| - Female root-knot nematode can present compounds able to activate defense mechanisms in the vegetable. With this end, the chromatographic technique is based on the purification of molecules such as proteins, glycoproteins, nucleic acids and polysaccharides, which may present eliciting characteristics. The aim of this paper was to determine the elicitors purification methodology, from phytopathogenic nematodes, by gel filtration chromatography (GFC). Nematodes females of Meloidogyne incognita and Meloidogyne javanica were extracted, macerated, and subsequently subjected to gel filtration chromatography. For the M. incognita five protein peaks and seven glucosidic peaks, with molecular masses ranging from 8.80 to 285.95 KDa and from 65.09 to 7.47 µg glucose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Meloidogyne incognita; Meloidogyne javanica; Eliciadores. |
| Ano: 2014 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/11175 |
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| Roncato, Sidiane Coltro; Stangarlin, José Renato; Flores, Éder Lisandro de Moraes; Gonçalves, Edilaine Della Valentina; Dildey, Omari Dangelo Forlin; Júnior, Affonso Celso Gonçalves. |
| The identification of molecules present in plants by chromatographic techniques such as HPLC, can bring promising alternative to the control of plant diseases, since brassicas have glucosinolates, which act in plant defense. The objective of this work was to verify the presence of allyl isothiocyanate by HPLC (High Performance Liquid Chromatography Reverse Phase), using different methods of extraction. The plant material was leaves of Crambe abyssinica, dried at 45 °C for 48 h. The extraction methods used consisted of 200 mg L-1 of plant material for each method: infusion, aqueous solution, and cetonic, hydroalcoholic, methanol, hexane and chloroform solutions. The identification and quantification were by HPLC on C18 column UV at 244 nm. The experiment... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crambe abyssinica Hochst; Glicosinolato; Cromatografia. |
| Ano: 2014 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/11174 |
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| Rissato, Bruna Broti; Stangarlin, José Renato; Coltro-Roncato, Sidiane; Dildey, Omari Dangelo Forlin; Gonçalves, Edilaine Della Valentina; Lorenzetti, Eloisa. |
| Sclerotinia sclerotiorum, the causal agent of white mold, is a polyphagous pathogen with a large number of plant hosts, and that can survival for many years in the soil. Alternative methods are necessary to its control, such as homeopathy. This work aimed to verify the antimicrobial activity of homeopathic drugs against S. sclerotiorum. Two assays were conducted: the first one using Nosode of S. sclerotiorum, and the second with Sulphur, both at 0, 6, 12, 24, 36 and 48 CH (hahnemanian centesimal dilution), and distillated water as control treatment. The dilution 0 CH was the hydro alcoholic solution with ethanol 30%. The number of sclerotia and the mycelial growth were evaluated in vitro. None of the drugs reduced the mycelial growth of S. sclerotiorum,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crescimento micelial; Homeopatia; Nosodio; Número de escleródios; Sulphur.. |
| Ano: 2016 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/13353 |
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| Broetto, Laline; Coltro, Sidiane; Meinerz, Cristiane Cláudia; Dildey, Omari Dangelo Forlin; Pazdiora, Paulo César; Gonçalves, Edilaine Della Valentina; de Moares, Alice Jacobus; Henkemeir, Nicanor Pilarski; Kuhn, Odair José; Stangarlin, José Renato. |
| Charcoal Rot is a disease that occurs in a large number of plant species and besides not being economically controlled by fungicides no genetic resistance has been found. Associated with this controlling problem, there is also a growing awareness of the proper handling of pesticides or of the search for new and environmentally safer technologies for handling of plant diseases. Therefore this study aimed to examine in vitro the potential of different isolates of Trichoderma sp for biological control of Macrophomina phaseolina. Seventeen different isolates of Trichoderma sp. were used, in two different tests: direct confrontation with the pathogen and production of volatile compounds by the antagonist. In the experimental conditions the different isolates of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Controle biológico. Confronto direto. Composto volátil. Podridão Carvão.. |
| Ano: 2014 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/7893 |
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